Food Pathogens

For the field of foodborne pathogens the focus will be on improving analytical speed and reliability for the detection and identification of bacteria that form a direct hazard to human health. Foodborne pathogens are usually heterogeneously distributed and present in low numbers. New and better sample preparation and DNA extraction methods will be developed in DECATHLON. These will permit analysis of much larger samples and minimize or circumvent current need for time consuming enrichment. The resolution and discriminatory power of current detection methods will be improved to increase detectability of emerging pathogens and better distinguish between hazardous bacteria and similar bacteria that are not pathogenic. DNA sequence markers for pathogenicity and strain typing will be identified through mining of the available, very large number of completely sequenced bacterial genomes and other genetic information in databases, literature and other open sources. These markers will be ranked according to relevance for the harmonised integration into all developed methodologies for foodborne pathogens.

List with primary targets to be used for EHEC detection and typing. The document summarizes three primary targets to be used for EHEC detection and subtyping including relevant reference bacterial isolates and the corresponding gene accession numbers.