D 6.6 - Minimum Performance Parameters for molecular analytical methods

This document contains proposals for definitions of minimum performance parameters (MPPs) and associated acceptance values (AAVs) for DNA extraction, nucleic acid amplification techniques and Next Generation Sequencing (NGS) analysis. Attention has been paid to minimize bias toward sectors represented in DECATHLON (foodborne pathogens, genetically modified organisms, and taxed products and endangered species). This is true for MPPs as well as for AAVs. The list of examples of MPPs is not exhaustive and yet includes MPPs that stakeholders (depending on sector and scope) may find superfluous. Some of the MPPs are potentially overlapping, for others the definitions and their AAVs fit to various degrees in different chapters dedicated to DNA extraction, nucleic acid amplification and NGS, respectively. Much attention has been paid to maximize harmonization and minimize repetition.